Nucleus Pulposus Cell Conditioned Medium Promotes Mesenchymal Stem Cell Differentiation into Nucleus Pulposus-Like Cells under Hypoxic Conditions

Figure 1

Markers recommended for defining the young healthy NP phenotype. Relative gene expression by qPCR in NPCs cultured under normoxic and hypoxic conditions. Gene expression levels for each sample were normalized against the housekeeping gene β-actin. (a) Aggrecan, (b) brachyury, (c) carbonic anhydrase 3, (d) carbonic anhydrase 12, (e) CD24, (f) collagen II, (g) Glut-1, (h) HIF-1α, (i) HIF-2α, (j) KRT8, (k) KRT18, (l) KRT19, and (m) Shh. , , , and .

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