Nucleus Pulposus Cell Conditioned Medium Promotes Mesenchymal Stem Cell Differentiation into Nucleus Pulposus-Like Cells under Hypoxic Conditions

Figure 5

(a–c) Western blotting assessment of relative protein expression in MSCs under normoxic and hypoxic conditions with the 50% or 100% NPC conditioned medium. Protein expression levels for each sample were normalized against the housekeeping gene β-actin. (d) Aggrecan: (i) normoxia, (ii) hypoxia; (e) brachyury: (i) normoxia, (ii) hypoxia; (f) collagen II: (i) normoxia, (ii) hypoxia; (g) KRT8: (i) normoxia, (ii) hypoxia; (h) KRT18: (i) normoxia, (ii) hypoxia; (i) KRT19: (i) normoxia, (ii) hypoxia; and (j) Shh: (i) normoxia, (ii) hypoxia. , , , and .

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