Nucleus Pulposus Cell Conditioned Medium Promotes Mesenchymal Stem Cell Differentiation into Nucleus Pulposus-Like Cells under Hypoxic Conditions

Table 2

Antibodies used in WB and IF.

Target	Antibody (company, order number)	WB usage	IF usage
HIF-1α	Abcam, ab2185	1 : 1000	1 : 1000
HIF-2α	Abcam, ab199	1 : 1000	1 : 1000
Glut-1	Abcam, ab15309	1 : 250	1 : 250
Shh	Abcam, ab19897	1 : 250	1 : 50
Brachyury	Bioss, bs-10669R	1 : 250	1 : 250
Aggrecan	Bioss, bs-11655R	1 : 250	1 : 250
Collagen II	Abcam, ab116242	1 : 1000	1 : 1000
Carbonic anhydrase 3	Abcam, ab196835	1 : 1000	1 : 100
Carbonic anhydrase 12	(D78E4) CST, 5865	1 : 1000
Carbonic anhydrase 12	Bioss, bs-6025R		1 : 250
CD24	Proteintech, 10600-1-AP	1 : 1000
CD24	Bioss, bs-4891R		1 : 250
Cytokeratin 8	Abcam, ab59400	1 : 1000	1 : 500
Cytokeratin 18	Abcam, ab181597	1 : 1000	1 : 100
Cytokeratin 19	Abcam, ab84632	1 : 500	1 : 500
β-Actin	Abcam, ab8227	1 : 2000

WB: western blotting; IF: immunofluorescence.