p130 And pRb in the Maintenance of Transient Quiescence of Mesenchymal Stem Cells

Figure 3

The analysis of the protein structure of the pocket proteins (pp)-E2f-DNA complexes in the T98G, HeLa, and the 293HEK cells by EMSA. (a) The proteins composing the pp-E2f-DNA complexes in the quiescent and cycling T98G cells. (b) The structure of the pp-E2f-DNA complexes in the cycling HeLa cells. (c) The pp-E2f-DNA complexes are completely absent in the 293HEK cells. The samples prepared from the cycling cells were treated with competitor—C, deoxycholate—D, or different antibodies as indicated. The cycling T98G, HeLa, and 293HEK cells were used, accordingly, in the 18 h and 12 h points after restimulation. The protein-DNA bands that included different proteins designated with (+), the band abolishment—(-), the supershifts induced by specific antibodies—(++), and nonspecific bands—().