Small Extracellular Vesicles from Human Fetal Dermal Cells and Their MicroRNA Cargo: KEGG Signaling Pathways Associated with Angiogenesis and Wound Healing

Figure 1

Physical characterization of EVs by NTA and protein expression. (a) Representative histogram of EVs isolated from secretome of fetal dermal cells showing a peak corresponding to a mode value of size and a concentration of particles/ml. (b) Representative histogram of EVs isolated from secretome of adult dermal cells showing a peak corresponding to a mode value of size and a concentration of particles/ml. The results shown are representative of three independent experiments. (c) Representative Western blot analysis of two fetal dermal-derived EV samples showing expression of EV markers Rab5, Alix, and CD63 in total protein extracts of pellet particles. Negative control, calnexin in cell protein extracts, and pellet particles are also shown. NTA: nanoparticle tracking analysis; EVs: extracellular vesicles; cells: total protein extracts of human fetal dermal cells; EV#1: sample 1; EV#2: sample 2.

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