Small Extracellular Vesicles from Human Fetal Dermal Cells and Their MicroRNA Cargo: KEGG Signaling Pathways Associated with Angiogenesis and Wound Healing

Figure 8

Internalization of CSFE-labeled EVs by targeted fibroblasts. (a) EVs analyzed by flow cytometry in a linear range for physical parameters FSC vs. SSC (forward scatter vs. side scatter) using Miltenyi beads as size marker. CSFE-labeled EVs and negative control, unlabeled EVs are also shown. Confocal images showing uptake of CSFE-labeled EVs by fibroblast target cells at 4- and 8-hour incubation times. (b) Bright field image merged with green (CSFE) and blue (DAPI) showing cytoplasmic localization of fluorescent signal at 4 hours of incubation. (c) Dual-channel confocal fluorescence showing cytoplasmic localization of fluorescent signal at 8 hours of incubation. The results shown are representative of three independent experiments. CSFE: carboxyfluorescein succinimidyl ester; EVs: extracellular vesicles; FL1: fluorescence 1. Scale bar: 5 μm.

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