Research Article
Isolation and Characterization of Multipotent Canine Urine-Derived Stem Cells
Figure 4
Osteogenic differentiation of urine-derived cells in vitro. (a) Osteogenic gene (Runx2, Bglap, SPP1) expression compared between osteogenic medium and its respective basal cultures. , . Runx2: Runt-related transcription factor 2; Bglap, bone γ-carboxyglutamate protein; Spp1, secreted phosphoprotein. (b) The Runx2 protein expression of the urine-derived cells after 7 days of osteogenic induction. Scale . (c) Alizarin Red staining of cells after 21 days in osteogenic or basal medium. Calcium nodules were seen in the osteogenic induced medium. Scale .
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