Ascorbic Acid, Inflammatory Cytokines (IL-1β/TNF-α/IFN-γ), or Their Combination’s Effect on Stemness, Proliferation, and Differentiation of Gingival Mesenchymal Stem/Progenitor Cells

Figure 2

Wnt/β-catenin pathway activation, MTT, and CFUs following ascorbic acid and inflammatory stimulation of G-MSCs: ELISA examination of the Wnt/β-catenin signaling pathway for total intracellular β-catenin following G-MSCs’ challenging by ascorbic acid and inflammation (a; box and whisker plots with medians/quartiles). Relative G-MSCs metabolic activity following ascorbic acid and inflammatory stimulation for 24 h and 72 h (b; box and whisker plots with medians/quartiles). CFUs-assay/CFUs’ numbers following G-MSCs’ stimulation via ascorbic acid and inflammation (c; box and whisker plots with medians/quartiles). Significant differences denoted with asterisks (, ; ; Wilcoxon-signed-rank-test). Abbreviations: CFUs: colony-forming units; β-catenin: total β-catenin.

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