Differentiation Potential of Early- and Late-Passage Adipose-Derived Mesenchymal Stem Cells Cultured under Hypoxia and Normoxia

Figure 1

Relative gene expression levels by qRT-PCR for cells cultured under hypoxia and normoxia. (a) qRT-PCR for pluripotency genes KLF4, NANOG, and POU5F1. (b) qRT-PCR for MSC differentiation genes; osteogenic markers COL1A1 and RUNX2, chondrogenic markers SOX9 and COL2A1, and adipogenic marker PPARG. Relative expression levels are expressed using the GAPDH as the reference gene, and values were normalized to P5H; error bars represent SD. Statistically significant difference, , shown by for relative expression level when compared to P5H sample.

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