Differentiation Potential of Early- and Late-Passage Adipose-Derived Mesenchymal Stem Cells Cultured under Hypoxia and Normoxia

Figure 3

Osteogenic differentiation of MSCs cultured under hypoxia and normoxia. Alizarin Red S staining for cells differentiated for 19 days imaged in (a–e) culture wells and (f–j) on coverslips. (a) Negative control, (b) passage 5 hypoxia (P5H), (c) passage 9 hypoxia (P9H), (d) passage 5 normoxia (P5N), and (e) passage 9 normoxia (P9H). Scale bar 100 μm.

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