Human Cardiac Fibroblast Number and Activation State Modulate Electromechanical Function of hiPSC-Cardiomyocytes in Engineered Myocardium

Figure 1

Human cardiac fibroblasts display classic signs of activation in monolayer culture. (a and b) HCFs cultured on soft 12.5 kPa polyacrylamide gels or stiff 50 GPa glass were stained for vimentin (Vim) and α-smooth muscle actin (αSMA (a)), and fluorescence intensity was quantified ((b) , ). (c and d) HCFs cultured on glass were stimulated with 10 ng/mL TGF-β1, stained (c), and quantified ((d) , ). (e and f) Early-passage (p4) and late-passage (p9) human cardiac fibroblasts (, ). Data are represented as .