The Fabrication and Evaluation of a Potential Biomaterial Produced with Stem Cell Sheet Technology for Future Regenerative Medicine

Figure 2

Evaluation of decellularized efficiency with repeated freeze-thaw, Triton X-100, and SDS decellularization. (a) H&E staining after the decellularization treatment. Compared with freeze-thaw treatments, SDS and Triton X-100 treatments removed the cellular contents more efficiently. The repeated freeze-thaw method with cell components residual, SDS method with disturbed and partial rupture collagen fiber, and decellularized Triton X-100 sample with an intact preserved collagen fiber structure. (b) Microstructural observations by scanning electron microscopy. (c) DNA quantification of different groups. Compared with the repeated freeze-thaw method, the methods of Triton X-100 and SDS more effectively reduced the DNA content (), but no statistically significant differences were found between these two groups ().

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