The Fabrication and Evaluation of a Potential Biomaterial Produced with Stem Cell Sheet Technology for Future Regenerative Medicine

Figure 4

Recellularization capacity of decellularized ADSC sheets in comparison to conventional silk fibroin. (a) Oral mucosal epithelial cell acquisition and culture. (b) Cell proliferation assessment by CCK-8 assay; the decellularized ADSC sheets exhibited a higher proliferative capacity than silk fibroin on days 1, 3, 5, and 7 after cell seeding, and the gap of absorbance value was widening with time; silk fibroin vs. DAS on day 3 after cell seeding (); silk fibroin vs. DAS on day 5 after cell seeding (); silk fibroin vs. DAS on day 7 after cell seeding (). (c) H&E staining observation after cell inoculation: ① silk fibroin recellularization at 3 days of culture, ② silk fibroin recellularization at 7 days of culture—the black box represents a high magnification photograph (400x), ③ decellularized ADSC sheets recellularization at 3 days of culture, and ④ decellularized ADSC sheet recellularization at 7 days of culture—the black box represents a high magnification photograph (400x). (d) SEM observation after cell inoculation: ① morphology of silk fibroin, ② morphology of silk fibroin recellularization at 3 days of culture, ③ morphology of silk fibroin recellularization at 7 days of culture, ④ morphology of decellularized ADSC sheets, ⑤ morphology of decellularized ADSC sheet recellularization at 3 days of culture, and ⑥ morphology of decellularized ADSC sheet recellularization at 7 days of culture. DAS = decellularized ADSC sheets.

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