Research Article
MEST Regulates the Stemness of Human Periodontal Ligament Stem Cells
Table 2
Primer sequences, annealing temperatures, cycle numbers, and product sizes for quantitative RT-PCR.
| | Gene (abbreviation) | Primer sequence forward/reverse | Annealing temperature (°C) | Quantitative RT-PCR cycles | Size of amplified products (bp) |
| | p75NTR | 5-CCGAGGCACCACCGACAACC-3/5-TGCTTGCAGCTGTTCCACCTCT-3 | 60 | 40 | 108 | | N-cadherin | 5-GAAAGACCCATCCACGCCGAGC-3/5-TCAGCCGCTTTAAGGCCCTCATT-3 | 60 | 40 | 101 | | NANOG | 5-TCCAACATCCTGAACCTCAGCTA-3/5-AGGTTCCCAGTCGGGTTCAC-3 | 60 | 40 | 193 | | MEST | 5-ATGGGCCATTGGATCCTGTA-3/5-CAGAAGGAGTTGATGAAGCCCA-3 | 60 | 40 | 169 | | CCND1 | 5-AGGAGAACAAACAGATCA-3/5-TAGGACAGGAAGTTGTTG-3 | 60 | 40 | 163 | | CCNE1 | 5-GCCAGCCTTGGGACAATAATG-3/5-CTTGCACGTTGAGTTTGGGT-3 | 60 | 40 | 104 | | ALP | 5-GGACCATTCCCACGTCTTCAC-3/5-CCTTGTAGCCAGGCCCATTG-3 | 60 | 40 | 137 | | Osterix | 5-GCCATTCTGGGCTTGGGTATC-3/5-GAAGCCGGAGTGCAGGTATCA-3 | 60 | 40 | 129 | | PPARγ | 5-TATTCTCAGTGGAGACCGCC-3/5-TGAGGACTCAGGGTGGTTCA-3 | 60 | 40 | 115 | | C/EBPα | 5-GGTGGACAAGAACAGCAAGGA-3/5-GTCATTGTCACTGGTCAGCTC-3 | 60 | 40 | 135 | | COL-2 | 5-CAACCAGGACCAAAGGGACA-3/5-ACCTTTGTCACCACGATCCC-3 | 60 | 40 | 129 | | Aggrecan | 5-TGGTGATGATCTGGCACGAG-3/5-CGTGAGCTCCGCTTCTGTAG-3 | 60 | 40 | 124 | | COL-1 | 5-CCCGGGTTTCAGAGACAACTTC-3/5-TCCACATGCTTTATTCCAGCAATC-3 | 60 | 40 | 148 | | PLAP-1 | 5-ATGGGAGTCTTGCTAACATACCAC-3/5-CAGAAGTCATTTACTCCCACTCTTG-3 | 60 | 40 | 154 | | POSTN | 5-CACAACCTGGAGACTGGACA-3/5-TGCTTCCTTGTGTGGTCTTT-3 | 60 | 40 | 198 | | β-Actin | 5-ATTGCCGACAGGATGCAGA-3/5-GAGTACTTGCGCTCAGGAGGA-3 | 60 | 40 | 89 |
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