lncRNA TUG1 Facilitates Colorectal Cancer Stem Cell Characteristics and Chemoresistance by Enhancing GATA6 Protein Stability

Figure 1

Regulation of lncRNA TUG1 on the characteristics of CRC stem cells. (a) Flow cytometry analysis of CD133⁺/CD44⁺ and CD133⁻/CD44⁻ cells sorted from colorectal cancer (CRC) cell lines HCT-116 and SW480. (b) Quantitative real-time PCR (qRT-PCR) was carried out to quantify the lncRNA TUG1 expression in CD133⁺/CD44⁺ and CD133⁻/CD44⁻ cells. (c) si-TUG1 and its control were transfected into SW480 and HCT-116 cells. The expression of lncRNA TUG1 was assessed by qRT-PCR. (d) The formation of clonal spheres was assessed by sphere formation assay (scale bar: 100 μm). (e) The protein levels of ALDH1 and Nanog in SW480 and HCT-116 cells were measured by Western blot. (f) pcDNA-TUG1 was transfected into SW480 and HCT-116 cells. qRT-PCR was conducted to quantify the lncRNA TUG1 expression. (g) The protein levels of ALDH1 and Nanog in SW480 and HCT-116 cells were detected using Western blot analysis. vs. CD133⁻/CD44⁻. vs. CD133⁻/CD44⁻, pc-NC, or si-NC. NC: negative control.

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