lncRNA TUG1 Facilitates Colorectal Cancer Stem Cell Characteristics and Chemoresistance by Enhancing GATA6 Protein Stability

Figure 4

Verification of the regulation of the lncRNA TUG1/GATA6 axis on the characteristics and chemoresistance of CRC stem cells. si-TUG1 and/or pcDNA-GATA6 were transfected into CD133⁺/CD44⁺ isolated from SW480 cells. (a) qRT-PCR and Western blot assays were carried out to identify the transfection efficiency of pcDNA-GATA6. (b) Analysis of the protein levels of ALDH1 and Nanog in CD133⁺/CD44⁺ isolated from SW480 cells using Western blot. (c) The formation of clonal spheres was assessed by sphere formation assay. si-TUG1 and/or pcDNA-GATA6 were transfected into CD133⁺/CD44⁺ isolated from SW480 cells, and the cells were then treated with 4 μM oxaliplatin for 48 h. (d) The viability of CD133⁺/CD44⁺ isolated from SW480 cells was analyzed using a CCK-8 assay. (e) Analysis of the apoptotic ability of CD133⁺/CD44⁺ isolated from SW480 cells by flow cytometry. vs. si-NC. vs. si-TUG1+pc-NC. ^# and ^### vs. si-TUG1+pc-NC.

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