lncRNA TUG1 Facilitates Colorectal Cancer Stem Cell Characteristics and Chemoresistance by Enhancing GATA6 Protein Stability

Figure 5

Regulation of lncRNA TUG1 on the CRC stem cell characteristics and chemoresistance in vivo. CD133⁺/CD44⁺ isolated from HCT-116 cells transfected with Lv-shTUG1 were injected into mice to establish a xenograft model and the oxaliplatin (5 mg/kg) was injected into mice every three days, and 6 mice were randomly assigned to each group. (a) The tumor volume of mice was monitored (). (b) Detection of the lncRNA TUG1 expression in tumor tissues by qRT-PCR. (c) Detection of the GATA6 mRNA level using qRT-PCR. (d) Analysis of the GATA6, BMP4, and BMP2 protein levels in tumor tissues by Western blot. (e) Detection of the protein levels of ALDH1 and Nanog using Western blot. (f) The cell apoptosis in tumor tissues was assessed by TUNEL assay (scale bar: 20 μm). and vs. Lv-shNC; ^### vs. Lv-shNC+OXA. OXA: oxaliplatin.

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