KIF2C Is a Novel Prognostic Biomarker and Correlated with Immune Infiltration in Endometrial Cancer

Figure 2

KIF2C expression is correlated with poor progress and outcomes in EC from the TCGA. (a, b) Knockdown the expression of KIF2C in Ishikawa and RL95-2 cells. (c) GSEA analysis suggests that KIF2C may be involved in some common signaling pathways, such as cell cycle, DNA replication, ERBB signaling pathway, oocyte meiosis, and ubiquitin-mediated proteolysis. (d) The cell cycle in Ishikawa and RL95-2 cells were analyzed by flow cytometry. (e–f) After being transfected with shNC and shKIF2C, the migration and invasion abilities of Ishikawa and RL95-2 cells were assessed by Transwell assay (). (g) The knockdown of KIF2C reduces the number of colonies in Ishikawa and RL95-2 cells (original magnification at ×100; error bars ; ). (h) CCK8 assays were performed to determine the effect of shKIF2C on cell growth in vitro. Scale bars, 200 μm; error bars ; ; ; .

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