KIF2C Is a Novel Prognostic Biomarker and Correlated with Immune Infiltration in Endometrial Cancer

Figure 5

Blockade of PD1 on CD8⁺ T cell immunity inhibits tumor growth in vivo. (a) Mice were injected with human Ishikawa cells transfected with shKIF2C or shNC, the entailed injections with CD8⁺ T cell or CD8⁺ T cell in combination with an intraperitoneal injection of an anti-PD1 antibody. A schematic representation of the treatment was shown. (b, c) Representative images of tumors and a comparison of Ishikawa tumor growth in different groups were shown ( per group). (d) The correlation between KIF2C and CD8⁺ T cell in xenograft. Results were expressed as log (ln) of integrated density in xenograft. Each dot in (d) represents 1 field. (e) Representative analysis of KIF2C (green), DAPI (blue), CD8⁺ T cell (red), and PDL1 (pink) in xenograft by multiplex fluorescent immunohistochemistry (original magnification at ×200). Scale bars, 50 μm. Error bars . ; ; ; .

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