Cloning and Embryo Splitting in Mammalians: Brief History, Methods, and Achievements

Table 2

Studies in livestock embryo splitting.

Species	Embryo sample	Method	Efficiency	Reference
Sheep	Two- & four-cell	Biopsy	36%	[36]
Bovine	Eight-cell	Biopsy	18%	[37]
Bovine	Morula	Bisection	66.6%	[38]
Bovine	Morula	Bisection	62.5%	[39]
Pig	Morula	Bisection	25%	[40]
Goat	Morula	Bisection	37%	[41]
Horse	Two- & eight-cell	Biopsy	50%	[42]
Bovine	Morula	Bisection	63.2%	[43]
Bovine	Morula	Bisection	53%	[44]
Goat	Morula	Bisection	59%	[45]
Sheep	Morula	Bisection	68%	[46]
Pig	Morula	Bisection	30%	[47]
Bovine	Morula	Bisection	74.6%	[48]
Sheep	Eight-cell	Biopsy	23.2%	[49]
Bovine	Morula	Bisection	69.2%	[50]