A Novel High-Content Screening-Based Method for Anti-Trypanosoma cruzi Drug Discovery Using Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Figure 2

Infection of hiPSC-CMs with T. cruzi and high-content screening analysis. (a) Representative image of uninfected hiPSC-CM control, showing cTnT staining (green) and nuclei stained with Draq5 (red). (b) Representative image of hiPSC-CMs infected with T. cruzi, showing cTnT staining (green) nuclei and amastigotes stained with Draq5 (red). (c) Standardization of image analysis in Operetta High Content Imaging System, illustrating the steps of nuclei identification (left), followed by cytoplasm delimitation (middle), and T. cruzi amastigote spot identification (right). Quantification of the number of amastigotes/cell (d), percentage of infection (e), and number of hiPSC-CMs (f) 72 h following infection in and . ; ; (). .

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