miR-370-3p as a Novel Biomarker Promotes Breast Cancer Progression by Targeting FBLN5

Figure 4

miR-370-3p targets and inhibits FBLN5 and activates the NF-κB signaling pathway. (a) Quantitative RT-PCR was used to detect the expression of FBLN5 in breast cancer cells and them transfected with MNC, 370-mimics, INC, and 370-inhibitor. , . (b) The binding sites of miR-370-3p seed region and 3-UTR of FBLN5 mRNA were predicted. (c) The luciferase reporter gene analysis was used to detect the effect of miR-370-3p on the stability of FBLN5 mRNA. . (d) Western blot was used to detect the expression of FBLN5 and p-p65 in breast cancer cells transfected with MNC, 370-mimics, INC, and 370 -inhibitor. , , .

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