p57 Suppresses the Pluripotency and Proliferation of Mouse Embryonic Stem Cells by Positively Regulating p53 Activation

Figure 5

p57 interacted with and contributed to the activations of p53 in mESCs. (a) Visualization of p57-p53 interaction in vivo by BiFC assay (). (b) Coimmunoprecipitation of p57 (Flag-p57) and p53. (c, d) Western blot and related densitometric analysis of p53 and its phosphorylation level at Ser 315 in control and p57 knockdown (shp57) mESCs. (e, f) Western blot and related densitometric analysis of p53 and its phosphorylation level at Ser 315 in control and p57-overexpressing (p57) mESCs. (g) Real-time PCR analyses of p57, Nanog, Oct4, Sox2, Pcna, Cyclin A, and Cyclin E expression levels of control () and p57-overexpressing (p57, ) mESCs treated with p53 inhibitor cultured for 48 h. (h, i) Western blot and related densitometric analysis of p57, p-p53, p53, NANOG, OCT4, SOX2, PCNA, Cyclin A, and Cyclin E expression levels of control and p57-overexpressing (p57) mESCs treated with p53 inhibitor cultured for 48 h.  μm. .

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