CircRNA FAT1 Regulates Osteoblastic Differentiation of Periodontal Ligament Stem Cells via miR-4781-3p/SMAD5 Pathway

Figure 1

Identification of PDLSCs and verification of PDLSC multidirectional differentiation ability. (a): (I) Primary cells migrated from PDL tissues on day 3, and 80% confluence was observed on day 12 (scale bar: 100 μm). (II, III) Cell colonies formed by PDLSCs observed in the dish and an amplified image of a representative colony captured by a microscope (scale bar: 100 μm). (b) Flow cytometry results showed that CD29, CD73, CD90, and CD105 were positive in PDLSCs. In addition, both CD45 and CD34 were negative. (c) Multiple differentiation potentials of PDLSCs (from left to right: osteogenesis, adipogenesis, chondrogenesis; scale bar: 100 μm). (d) Immunofluorescence assay revealed that cultured PDLSCs were positive for STRO-1 (scale bar: 100 μm).

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