CircRNA FAT1 Regulates Osteoblastic Differentiation of Periodontal Ligament Stem Cells via miR-4781-3p/SMAD5 Pathway

<div>circFAT1 does not affect PDLSCs proliferation. (a) Cell proliferation capability influenced by circFAT1 was detected at 450 nm with CCK-8 assay. (I): CCK-8 results showed that circFAT1 had no significant effect on the proliferation of PDLSCs; (II): EdU assay further confirmed that circFAT1 had no significant effect on the proliferation of PDLSCs. (b) EdU-positive PDLSCs influenced by circFAT1 were detected by EdU kit (scale bar: 200 <i>μ</i>m). EdU (red), Hoechst333 (blue). (c) PCR gel electrophoresis verified that the primers were located at a single band of 449 kb. The cyclization site of circFAT1 was determined by plasmid vector construction. (d) RNA nucleocytoplasmic isolation experiments verified that circFAT1 was mainly located in the cytoplasm of cells. <span class="nowrap"><svg height="10.7395pt" id="M8" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -10.1025 50.9184 10.7395" width="50.9184pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 54C322 63 340 82 350 102C330 152 324 179 321 236C368 205 390 186 422 144C444 143 470 148 486 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Stem Cells International

fig2

Figure 2

Figure 2: CircRNA FAT1 Regulates Osteoblastic Differentiation of Periodontal Ligament Stem Cells via miR-4781-3p/SMAD5 Pathway 