CircRNA FAT1 Regulates Osteoblastic Differentiation of Periodontal Ligament Stem Cells via miR-4781-3p/SMAD5 Pathway

<div>Overexpression of miR-4781-3p decreases osteoblastic differentiation tendency of PDLSCs. (a)–(c) The results of Western blot and RT-qPCR showed SMAD5 and osteogenic markers (RUNX2, OSX, COL1A, and COL3A) were downregulated in miR-4781-3p mimic transfected PDLSCs (mimics group). In addition, RT-qPCR results showed that the expression of <i>circFAT1</i> was downregulated, and <i>miR-4781-3p</i> was upregulated in the mimics group. (d, e) Overexpression of miR-4781-3p reduced the ALP activity of PDLSCs 7 days after osteogenic induction. ARS showed the mineralization of PDLSCs was decreased significantly in the mimic group after osteogenic induction for 14 days. 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Stem Cells International

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Figure 5

Figure 5: CircRNA FAT1 Regulates Osteoblastic Differentiation of Periodontal Ligament Stem Cells via miR-4781-3p/SMAD5 Pathway 