CircRNA FAT1 Regulates Osteoblastic Differentiation of Periodontal Ligament Stem Cells via miR-4781-3p/SMAD5 Pathway

<div>Knockdown of miR-4781-3p increases osteoblastic differentiation tendency of PDLSCs. (a)–(c) The results of Western blot and RT-qPCR showed SMAD5 and osteogenic markers (RUNX2, OSX, COL1A, and COL3A) were upregulated in the miR-4781-3p inhibitor transfected PDLSCs (inhibitor group). In addition, RT-qPCR results showed that the expression of <i>circFAT1</i> was upregulated, and <i>miR-4781-3p</i> was downregulated in the inhibitor group. (d, e) Knockdown of miR-4781-3p increased the ALP activity of PDLSCs 7 days after osteogenic induction. ARS showed the mineralization of PDLSCs was added significantly in the inhibitor group after osteogenic induction for 14 days. 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Stem Cells International

fig6

Figure 6

Figure 6: CircRNA FAT1 Regulates Osteoblastic Differentiation of Periodontal Ligament Stem Cells via miR-4781-3p/SMAD5 Pathway 