CircRNA FAT1 Regulates Osteoblastic Differentiation of Periodontal Ligament Stem Cells via miR-4781-3p/SMAD5 Pathway

Figure 7

circFAT1 acting as a miRNA sponge for miR-4781-3p by targeting SMAD5. (a) I: Dual-luciferase reporter gene assay was used to verify the binding sites between circFAT1 and miR-4781-3p. II: Dual-luciferase reporter gene assay was used to verify the binding sites between SMAD5 and miR-4781-3p. (b) Rescue experiment results showed that miR-4781 inhibitor could reverse the inhibition of circFAT1 siRNA on the osteogenic ability of PDLSCs. The expression level of SMAD5 was also reversed by miR-4781 inhibitor. (c) After seven days, less expression of ALP was found in the si-FAT1 group and si-FAT1 + miR-4781 iNC group, while the expression of ALP increased in the si-FAT1 + inhibitor group. The mineralization nodules in the si-FAT1 group and si-FAT1 + miR-4781 iNC group decreased. (d) I: ALP activity showed the same trend. II: Mineralized nodules were significantly increased in the si-FAT1 + inhibitor group, and calcium content was significantly increased (). (e) FISH experiments proved that circFAT1 were mostly located in the cytoplasm, 18S and U6 were the internal control. (Scale bar: 25 μm). , , and .

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