Pluripotency-State-Dependent Role of Dax1 in Embryonic Stem Cells Self-Renewal

Figure 3

Properties of Dax1KO-ESCs in 2i/LIF and LIF/serum culture. (a) Morphology of colonies formed by the indicated cell lines. Cells were grown in 2i/LIF or LIF/serum for 5 days. Scale bar, 100 μm. (b) AP staining of colonies formed by the indicated cell lines. Cells were grown in 2i/LIF or LIF/serum for 5 days. Scale bar, 100 μm. (c) Percentage of colony types formed by cells is shown. Diff.: differentiated; Undiff.: undifferentiated. (d) Total cell number of the indicated lines cultured for multiple passages. (e) Cell cycle profiling of the indicated cell lines by FACS with propidium iodide (PI) staining and proportions of the cells at each cell cycle were shown in the graph. (f) The apoptosis rate of Dax1KO-ESCs cultured in the indicated conditions. Exponentially growing cells were analyzed for apoptosis using Annexin-V and propidium iodide. Data in (c)–(f) are represented as ; .

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