Interleukin-20 Acts as a Promotor of Osteoclastogenesis and Orthodontic Tooth Movement

<div>IL-20 can induce BMSCs to regulate the expression of OPG and RANKL and then influence osteoclastogenesis through the OPG/RANKL/RANK axis. (a) BMSCs were transfected with siRNA carrying Cy3 dye and imaged with a fluorescence-inverted microscope to confirm that siRNA had been transfected into the cells. (b and c) Western blotting analysis verified the inhibition of IL-20 and IL-20RB expression by siRNA and the overexpression of IL-20 using a plasmid. (d and e) Western blotting analysis detected protein levels of OPG/RANK/RANKL axis components and a subset of RANKL-mediated downstream signalling pathway components in BMMs cultured with BMSC CM treated with IL-20 siRNA, IL-20RB siRNA, and an IL-20 overexpression plasmid. 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Stem Cells International

fig5

Figure 5

Figure 5: Interleukin-20 Acts as a Promotor of Osteoclastogenesis and Orthodontic Tooth Movement 