RIPK1 Coordinates Bone Marrow Mesenchymal Stem Cell Survival by Maintaining Mitochondrial Homeostasis via p53

Figure 3

Effect of RIPK1 on mitochondrial homeostasis of bone marrow MSCs. (a) After 72 h transfection with siRNA in bone marrow MSCs, the opening of mPTP was analyzed by the flow cytometer using mPTP Assay Kit. Representative graphs illustrated the increase of mPTP opening after knockdown of RIPK1. The quantitative mPTP in bone marrow MSCs was demonstrated by RFI. Data are expressed as the from three independent experiments (, vs. siControl; ). (b) Representative graphs illustrated the loss of MMP after knockdown of RIPK1. The quantitative MMP in bone marrow MSCs was demonstrated by red/green ratio. Data are presented as the from three independent experiments (, vs. siControl; ). (c) Representative confocal images were obtained in situ staining with MitoTracker (mitochondria, red) and PicoGreen (DNA, green). Knockdown of RIPK1 promotes mitochondrial DNA release into the cytoplasm ( μm). (d) Representative western blots showing the release of cytochrome c. Histogram analysis showing the relative protein levels of cytochrome c. Data are presented as the from three independent experiments (, vs. siControl; ). (e) Representative western blots of the expression of PUMA. Histogram analysis showing the relative protein levels of PUMA. Data are presented as the from three independent experiments (, vs. siControl; ).

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