miR-302d Competitively Binding with the lncRNA-341 Targets TLE4 in the Process of SSC Generation

Figure 5

Screening and verification of target gene TLE4. (a) Venn diagram of biometric prediction software analysis result; detail information and 3UTR sequence of TLE4. (b) Expression level of miR-302d and TLE4 in ESCs, PGCs, and SSCs. (c) Dual-luciferase reporting assay; G1: pmiR-tle4-wt group (use TLE4 3UTR wild-type pmiR-REPORT vector to transfect DF-1 cells), G2: pmiR-tle4-wt+miR-302d mimics group (use TLE4 3UTR wild-type pmiR-REPORT vector and miR-302d mimics to transfect DF-1 cells), G3: pmiR-tle4-mt+miR-302d mimics group (use TLE4 3UTR mutant-type pmiR-REPORT vector and miR-302d mimics to transfect DF-1 cells, TLE4 3UTR mutant-type vector lacks binding site: AGCACTT).

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