Research Article
Extracellular Vesicles Released from Neprilysin Gene-Modified Human Umbilical Cord-Derived Mesenchymal Stem Cell Enhance Therapeutic Effects in an Alzheimer’s Disease Animal Model
Figure 9
Identification of migration of hUC-MSC-released EVs. (a) Representative image showing colocalization of CD63 and CD81 (human specific)-positive hUC-MSC-released EVs in subregions of the hippocampus. μm. (b) The graph shows the number of colocalized CD63 and CD81-positive hUC-MSC-released EVs in each group. CD63 and CD81-positive EVs were not detected in normal and Aβ1-42 injection groups, but only detected in naïve and NEP-enhanced hUC-MSC groups. EVs mainly migrated to the hippocampus than to the cortex and basal ganglia. The data represent and were analyzed by a -test. and comparison between hippocampus subregion and cortex; # and ## comparison between hippocampus subregion and BG. SEM: standard error of the mean; hUC-MSC: human umbilical cord-derived mesenchymal stem cell; Aβ: Aβ1-42 injection group; MSC: naïve hUC-MSC injection group; NEP-MSC: neprilysin-enhanced hUC-MSC injection group; DG: dentate gyrus; BG: basal ganglia.
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