Intranasally Administered L-Myc-Immortalized Human Neural Stem Cells Migrate to Primary and Distal Sites of Damage after Cortical Impact and Enhance Spatial Learning

Figure 1

TBI-specific distribution of intranasally delivered LM-NSC008 cells in rats (day 25). (a) Confocal image (stitched at maximum intensity) of optically cleared coronal brain slice (1 mm thick). Rats that received either a TBI or sham injury and on days 7, 9, 11, 13, 15, and 17 post-surgery were IN-administered LM-NSC008 cells ( cells in 24 μL). (a–c) On day 25, the rats were euthanized and 1 mm brain slices were cleared using CLARITY (PACT) and imaged using confocal microscopy. Three coronal sections (S1, S2, and S3) are shown overlapping the TBI site. LM-NSC008 cells were lentivirally transduced to express eGFP.Ffluc protein for visualization (LM-NSC008 cells—green and red arrows). LM-NSC008 cells highlighted and quantified within boxes 1–7 (the pseudo-object was drawn at TBI site A).