Research Article
Proteomic Analysis of Hypoxia-Induced Senescence of Human Bone Marrow Mesenchymal Stem Cells
Figure 2
Hypoxia-induced cell senescence at different time points and surface marker profiles. (a) Representative images of senescent hBMSCs using SA-β-gal staining. Blue: SA-β-gal-positive cells. . (b) Quantification of SA-β-gal-positive cells. The percentage of SA-β-gal-positive cells was significantly increased after hypoxia treatment, compared to the control group. 0 h, 4 h, and 12 h: time of hypoxia treatment. Experiments were performed in three hBMSC cultures from three different donors. . vs. the 0 h control group. (c) The expressions of surface markers such as CD34, CD117, CD45, CD29, CD44, CD105, CD73, and CD11b were not significantly different between the hypoxia-induced group and the control group.
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