Proteomic Analysis of Hypoxia-Induced Senescence of Human Bone Marrow Mesenchymal Stem Cells

Figure 5

KEGG pathway analysis of hypoxia-induced hBMSCs. (a) KEGG pathway enrichment analysis of differentially expressed proteins from cells treated with or without hypoxia. The upregulated (red) proteins were mainly involved in cytokine-cytokine receptor interactions and complement and coagulation cascades, whereas the downregulated (green) proteins were mainly involved in cancer pathways, the PI3K-Akt signaling pathway, proteoglycans in cancer, hepatocellular carcinoma, and cellular senescence. (b) KEGG pathway annotation statistics of differentially expressed proteins (top 20). The signaling pathway annotations of differentially expressed proteins were critically related to pathways in cancer (34 proteins), Alzheimer disease (25 proteins), amyotrophic lateral sclerosis (24 proteins), the PI3K-Akt signaling pathway (23 proteins), and Huntington disease (23 proteins). (c) The role of the CDK cluster and CCND1 in the PI3K signaling pathway. (d) The role of the CDK cluster in the p53 signaling pathway. (e) PPI network of differentially expressed proteins in the 12-hour hypoxia-induced group. PPI analyses indicated that CDK1, CDK2, and CCND1 were important nodes. STRING network bonds. Blue: from curated databases; rose pink: experimentally determined; green: gene neighborhood; red: gene fusions; navy blue: gene co-occurrence; yellow: text mining; black: coexpression; purple: protein homology.

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