Positively Correlated CD47 Activation and Autophagy in Umbilical Cord Blood-Derived Mesenchymal Stem Cells during Senescence

<div>Screening results revealed different CD47 expressions on two groups, depending on cellular proliferation. (a) Comparative results of cumulative population doubling (CPD) at passage 5 showed higher proliferative capacity on Group 2. (b) Screening for cell surface proteins between two groups was shown with heat map analysis based on 242 cell surface antibody screening. The significant difference in cell surface antigen expression between two groups was revealed: CD47, CD146, CD49b, CD274, and EGFR. (c, d) Flow cytometry identified two CD markers (CD146 and CD47), showing significant difference expression. (e) Immunoblotting results demonstrated that the expression of autophagy-related proteins, ATG5, ATG12, Beclin1, and LC3B, downregulated on Group 1. 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Stem Cells International

fig1

Figure 1

Figure 1: Positively Correlated CD47 Activation and Autophagy in Umbilical Cord Blood-Derived Mesenchymal Stem Cells during Senescence 