Dental Pulp Mesenchymal Stem Cells Attenuate Limb Ischemia via Promoting Capillary Proliferation and Collateral Development in a Preclinical Model

Figure 2

The blood perfusion and angiogenesis at ischemic limb after treatments with MSCs. Immediately after ligation and at 1, 2, and 3 weeks after cell transplantation, the blood perfusion was measured at the proximal side of ligation by laser Doppler perfusion imaging. The perfusion ratios of the ischemic limb : nonischemic limb were calculated and are presented in (a), and the representative images are shown in (b). At 4 weeks after MSC transplantation, the left femoral artery was separated from the groin to the knee joint and the proximal side was ligated. An artery catheter was inserted into the femoral artery from the distal side. Immediately after 0.6 to 0.8 ml iodoprolamine injection, the vascular network was observed through a continuous photograph by X-ray. The representative images are presented in (c). Data in (a) were shown as . , compared with the corresponding group.

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(b)

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