lncRNA HHIP-AS1 Promotes the Osteogenic Differentiation Potential and Inhibits the Migration Ability of Periodontal Ligament Stem Cells

<div>HHIP-AS1 knockdown inhibited the osteogenic differentiation potential of PDLSCs. (a) Real-time RT-PCR result showed the expression level of HHIP-AS1 at 0, 2, 4, and 6 h under continuous compressive pressure. (b) Real-time RT-PCR result showed the knockdown efficiency of HHIP-AS1 in PDLSCs. (c) ALP activity after 5 days of osteogenic induction. (d) Alizarin Red staining result after 2 weeks of osteogenic induction. (e) Calcium quantitative analysis. (f) Western blot results displayed expressions of BSP, OCN, and OSX after 1 week of osteogenic induction. GAPDH was used as internal control in real-time RT-PCR and Western blot. One-way ANOVA or Student’s <span class="nowrap"><svg height="8.02022pt" id="M16" style="vertical-align:-0.2063999pt" version="1.1" viewbox="-0.0498162 -7.81382 4.54925 8.02022" width="4.54925pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M324 430H196L233 583L223 592L145 529L120 430H54L29 396L31 388H111L56 126C33 15 54 -12 77 -12C137 -12 214 57 250 95L233 119C208 92 155 59 138 59C126 59 120 70 131 125L186 390L298 394L324 430Z"></path></g></svg>-</span>test was performed to determine statistical significance. 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Stem Cells International

fig1

Figure 1

Figure 1: lncRNA HHIP-AS1 Promotes the Osteogenic Differentiation Potential and Inhibits the Migration Ability of Periodontal Ligament Stem Cells 