MicroRNA Hsa-Let-7b Regulates the Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Targeting CTHRC1

Figure 1

Identification of PDLSCs. (a) Imaging examination of immature permanent teeth. (b) Scraping the middle third of the root of the tooth. (c) The morphology of primary PDLSCs (). (d) Morphology of the third-generation PDLSCs (). (e) Flow cytometry assay showed that PDLSCs were positive for CD29, CD73, CD90, and CD105, but negative for CD34 and CD45. (f) Immunofluorescence assay revealed that PDLSCs were positive for STRO-1 (). (g, h) PDLSCs had the potential to differentiate into osteoblasts and adipocytes (). On the left is the control group which was induced by complete medium. (i) PDLSCs had the potential to differentiate into chondrocytes ().

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)