MicroRNA Hsa-Let-7b Regulates the Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Targeting CTHRC1

Figure 3

Hsa-let-7b inhibited the osteogenic differentiation of PDLSCs. (a, b) Results of ALP staining and ALP activity assay after 7 days of osteogenesis (). . (c, d) ARS staining and CPC assay at day 14 after osteogenic induction (). . (e) The expression of osteogenesis-related proteins (ALP, RUNX2, and OSX) after osteogenic induction 3 days of transfected PDLSCs. GAPDH served as the reference protein. (f) Relative grayscale analyses of (e). . (g) The expression of osteogenesis-related genes (ALP, RUNX2, and OSX) in group mimic NC, mimic, inhibitor NC, and inhibitor at day 3, respectively. . (h, i) The immunofluorescence staining of RUNX2 and ALP in transfected PDLSCs and matched controls ().

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