MicroRNA Hsa-Let-7b Regulates the Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Targeting CTHRC1

Figure 5

CTHRC1 is a direct target of hsa-let-7b. (a) The wild-type CTHRC1-3-UTR (WT) and mutant CTHRC1-3-UTR reporter plasmid (MUT) were constructed. (b) Luciferase reporter assays in 293 T illustrated that hsa-let-7b could bind with CTHRC1. (c–e) Western blot and qRT-PCR analysis were performed to detect the protein and mRNA levels of CTHRC1 in group mimic NC, mimic, inhibitor NC, and inhibitor at day 3, respectively. . (f) The immunofluorescence staining of CTHRC1 in transfected PDLSCs and matched controls ().

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