Metformin-Induced MicroRNA-34a-3p Downregulation Alleviates Senescence in Human Dental Pulp Stem Cells by Targeting CAB39 through the AMPK/mTOR Signaling Pathway

<div>Metformin-induced miR-34a-3p downregulation suppresses senescence in DPSCs through the AMPK/mTOR signaling pathway. Aging DPSCs were transfected with miR-34a-3p mimics or miR-NC for 48 h and treated with 100 <i>μ</i>M metformin for 48 h. (a) SA-<i>β</i>-gal staining to determine senescence. Representative images are shown (scale bar: 20 <i>μ</i>m). (b) Quantitative analysis of SA-<i>β</i>-gal-positive cell rates. (c) Representative blots and (d) quantitative analysis of the expression levels of p53, p21, and p16 in aging DPSCs. (e) Representative blots of the expression levels of p-AMPK, AMPK, p-mTOR, and mTOR in aging DPSCs. (f) Quantitative analysis of p-AMPK/AMPK and p-mTOR/mTOR ratios. 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Stem Cells International

fig6

Figure 6

Figure 6: Metformin-Induced MicroRNA-34a-3p Downregulation Alleviates Senescence in Human Dental Pulp Stem Cells by Targeting CAB39 through the AMPK/mTOR Signaling Pathway 

Figure 6 | Metformin-Induced MicroRNA-34a-3p Downregulation Alleviates Senescence in Human Dental Pulp Stem Cells by Targeting CAB39 through the AMPK/mTOR Signaling Pathway 