Metformin-Induced MicroRNA-34a-3p Downregulation Alleviates Senescence in Human Dental Pulp Stem Cells by Targeting CAB39 through the AMPK/mTOR Signaling Pathway

<div>CAB39 alleviates miR-34a-3p-induced senescence in DPSCs through the AMPK/mTOR signaling pathway. Aging DPSCs were pretransfected with miR-34a-3p mimics or miR-NC for 5 h, prior to transfection with Lenti-CAB39 or vehicle control for 48 h. (a) Representative images of SA-<i>β</i>-gal staining and (b) quantitative analysis of SA-<i>β</i>-gal-positive cells (scale bar: 20 <i>μ</i>m). (c) Representative blots and (d) quantitative analysis of the expression levels of p53, p21, and p16. (e) Representative blots of the expression levels of p-AMPK, AMPK, p-mTOR, and mTOR. (f) Quantitative analysis of p-AMPK/AMPK and p-mTOR/mTOR ratios. 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Stem Cells International

fig7

Figure 7

Figure 7: Metformin-Induced MicroRNA-34a-3p Downregulation Alleviates Senescence in Human Dental Pulp Stem Cells by Targeting CAB39 through the AMPK/mTOR Signaling Pathway 

Figure 7 | Metformin-Induced MicroRNA-34a-3p Downregulation Alleviates Senescence in Human Dental Pulp Stem Cells by Targeting CAB39 through the AMPK/mTOR Signaling Pathway 