Stromal Vascular Fraction and Amniotic Epithelial Cells: Preclinical and Clinical Relevance in Musculoskeletal Regenerative Medicine
Table 5
In vitro, in vivo, and clinical studies on AECs in cartilage regeneration.
Experimental model
Treatment groups
Evaluations
Results
Ref
In vitro: Cartilage samples with defect of 2 mm Human chondrocytes from femoral heads or knee () hBMSCs from femoral heads of donors (50-70 yrs) hAECs hAMSC HAM scaffold
Group 1: scaffold seeded with chondrocytes+chondrocyte pellet () Group 2: scaffold seeded with BMSCs+BMSC pellet () Group 3: scaffold seeded with AECs+AEC pellet () Group 4: scaffold seeded with AMSCs+AMSC pellet ()
Histology IHC (COLL I, COLL II)
2 mo Group 1: =repair of lesioned area, ICRS score than groups 2, 3, and 4 Group 4: ↑ COLL II protein than group 1 Group 2: ↑ COLL I protein than groups 1, 3, and 4
Group 1: micromasses+TGFβ1 (1 ng/ml) Group 2: micromasses+BMP7 (100 ng/ml)
RT-PCR (SOX9, COLL II, PG)
3 days Group 2: ↑ SOX9, COLL II gene expression than group 1 3 wks Group 1: ↑ SOX9, COLL II gene expression than group 2 Groups 1 and 2: ↑ PG gene expression during time
