miR-375 Promotes Pancreatic Differentiation In Vitro by Affecting Different Target Genes at Different Stages

Figure 2

The effects of miR-375 overexpression and inhibition during differentiation of human embryonic stem cells to insulin-producing cells. (a) Illustration of the transfection process. We used liposomes to perform transfection of miR-375 mimics and miR-375 inhibitors at the beginning of each phase (D0, D4, D7, D11, D14, and D18). Samples were collected at the end of each stage (D3, D6, D10, D13, D17, and D21). (b) The effect of the miR-375 mimic and miR-375 inhibition on transcription factors. (c) Western blotting detection and grayscale analysis were used to determine the protein expression of PDX-1 (D13) and INSULIN (D21) after miR-375 overexpression and inhibition.

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