CGF Membrane Promotes Periodontal Tissue Regeneration Mediated by hUCMSCs through Upregulating TAZ and Osteogenic Differentiation Genes

Figure 6

The effects of CGF fibrin membrane, pc-TAZ, and shTAZ on the nuclear transfer of TAZ, cell viability, and migration. (a and b) TAZ protein expression in cytoplasm and nucleus was detected by Western blot. β-Actin and Lamin B1 were employed as an internal control. (c) Cell viability was measured by MTT assay. (d) Cell migration was detected by Scratch assay, under 200 × magnification. All experiments were performed in independent triplicate. , , vs. blank; ^#, ^##, ^### vs. CGF; ^{^}, ^{^^}, ^{^^^} vs. vector; ^&, ^&&& vs. CGF + vector; ^△, ^△△△ vs. shTAZ; ^§, ^§§§ vs. pc-TAZ.

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