CGF Membrane Promotes Periodontal Tissue Regeneration Mediated by hUCMSCs through Upregulating TAZ and Osteogenic Differentiation Genes

Figure 8

The effects of CGF fibrin membrane, pc-TAZ, and shTAZ on the expression of cocultured osteogenesis-related genes and fibrosis-related genes. (a–c) The mRNA expressions of osteogenesis-related genes were analyzed by qRT-PCR. β-Actin was employed as an internal control. (d) Protein expressions of fibrosis-related genes were detected by Western blot. β-Actin was employed as an internal control. (e) The mRNA expressions of fibrosis-related genes were analyzed by qRT-PCR. β-Actin was employed as an internal control. All experiments were performed in independent triplicate. , , vs. blank; ^#, ^##, ^### vs. CGF; ^{^}, ^{^^}, ^{^^^} vs. vector; ^&, ^&&, ^&&& vs. CGF + vector; ^△, ^△△ vs. shTAZ; ^§, ^§§ vs. pc-TAZ.

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