Discovery of a Novel Long Noncoding RNA Lx8-SINE B2 as a Marker of Pluripotency

Figure 1

Mapping the full-length sequence of lncRNA Lx8-SINE B2. (a) Schematic of the 3-rapid amplification of cDNA ends (RACE) (left) and 3 RACE result for lncRNA Lx8-SINE B2 (right). (b) Schematic of the 5 RACE and its result for lncRNA Lx8-SINE B2. (c) DNA sequencing of RACE using a universal primer in pEASY-T1 vector. (d) Validation of lncRNA Lx8-SINE B2 transcript size by PCR from cDNA. M, DNA marker.

(a)

(b)

(c)

(d)