Discovery of a Novel Long Noncoding RNA Lx8-SINE B2 as a Marker of Pluripotency

Figure 2

Genomic location and expression of lncRNA Lx8-SINE B2. (a) Schematic of the mouse lncRNA Lx8-SINE B2 locus on chromosome 13. lncRNA Lx8-SINE B2 is between Adgrv1 and Lysmd3. There are three exons and some retrotransposon fragments of LINE or SINE in lncRNA Lx8-SINE B2. RT–qPCR primers were indicated below. (b) The expression level of lncRNA Lx8-SINE B2, Oct4 Sox2, and Esrrb in mESCs in the presence or absence of LIF for 2-4 days, as measured by RT–qPCR and normalized to Gapdh levels. Biological-triplicate data ( dishes) are presented as . (c) qPCR analysis of the expression level of lncRNA Lx8-SINE B2 in mouse ESCs and MEF cells. according to two-sided Student’s -test (d) Expression analysis of lncRNA Lx8-SINE B2 in ESCs cultured under serum/LIF, 2i/LIF or 2i condition. Biological-triplicate data ( extracts) are presented as .

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